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tgn46 rrid ab 324049  (Bio-Rad)


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    Bio-Rad tgn46 rrid ab 324049
    Golgi organization is altered upon loss of GMAP210 or Golgin-160. (A and C) Maximum projection confocal images of WT and GMAP210 KO (A) and Golgin-160 KO (C) RPE1 cells immunolabeled for cis -Golgi (GM130, magenta), cis/medial -Golgi (giantin, green), and TGN <t>(TGN46,</t> blue) markers. Nuclei labeled with DAPI (grayscale). Scale bar, 10 µm. Inset scale bar, 1 µm. (B and D) Quantification of total giantin and <t>TGN46</t> area and fragment number per cell from images represented in A and C. Individual dots represent one cell and are colored by replicate ( n = 3). Bars show the median from each replicate experiment. Statistical analysis was performed using a Shapiro–Wilk normality test and a Kruskal–Wallis significance test. (E–G) Tomographic reconstructions of Golgi structures in WT (E), GMAP210 KO (F), and Golgin-160 KO (G) cells. Segmented membranes are labeled as cisternae (blue/purple), dilated structures (red), tubulovesicular structures (yellow), and vesicles (green). (E ii, F ii, iii, and G ii iii) Single-slice images with segmentation. (F ii and iii) Open arrows point to invaginations within spherical regions of tubulovesicular structures. (G ii and iii) Double-headed arrows indicate top-to-bottom fenestrations in cisternae, closed-headed arrows indicate budding structures at the nuclear envelope, and pink arrow shows frustrated budding/fusion intermediate. (E i, iii, iv, F i, iv, v, and G i, iv, v) 3D rendering of segmentation. (E–G i) Scale bar, 1 µm.
    Tgn46 Rrid Ab 324049, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 755 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tgn46+rrid+ab+324049/Sheep+anti+Human+TGN46/pmc12360289-38-0-3
    Average 96 stars, based on 755 article reviews
    tgn46 rrid ab 324049 - by Bioz Stars, 2026-09
    96/100 stars

    Images

    1) Product Images from "Multiple golgins are required to support extracellular matrix secretion, modification, and assembly"

    Article Title: Multiple golgins are required to support extracellular matrix secretion, modification, and assembly

    Journal: The Journal of Cell Biology

    doi: 10.1083/jcb.202411167

    Golgi organization is altered upon loss of GMAP210 or Golgin-160. (A and C) Maximum projection confocal images of WT and GMAP210 KO (A) and Golgin-160 KO (C) RPE1 cells immunolabeled for cis -Golgi (GM130, magenta), cis/medial -Golgi (giantin, green), and TGN (TGN46, blue) markers. Nuclei labeled with DAPI (grayscale). Scale bar, 10 µm. Inset scale bar, 1 µm. (B and D) Quantification of total giantin and TGN46 area and fragment number per cell from images represented in A and C. Individual dots represent one cell and are colored by replicate ( n = 3). Bars show the median from each replicate experiment. Statistical analysis was performed using a Shapiro–Wilk normality test and a Kruskal–Wallis significance test. (E–G) Tomographic reconstructions of Golgi structures in WT (E), GMAP210 KO (F), and Golgin-160 KO (G) cells. Segmented membranes are labeled as cisternae (blue/purple), dilated structures (red), tubulovesicular structures (yellow), and vesicles (green). (E ii, F ii, iii, and G ii iii) Single-slice images with segmentation. (F ii and iii) Open arrows point to invaginations within spherical regions of tubulovesicular structures. (G ii and iii) Double-headed arrows indicate top-to-bottom fenestrations in cisternae, closed-headed arrows indicate budding structures at the nuclear envelope, and pink arrow shows frustrated budding/fusion intermediate. (E i, iii, iv, F i, iv, v, and G i, iv, v) 3D rendering of segmentation. (E–G i) Scale bar, 1 µm.
    Figure Legend Snippet: Golgi organization is altered upon loss of GMAP210 or Golgin-160. (A and C) Maximum projection confocal images of WT and GMAP210 KO (A) and Golgin-160 KO (C) RPE1 cells immunolabeled for cis -Golgi (GM130, magenta), cis/medial -Golgi (giantin, green), and TGN (TGN46, blue) markers. Nuclei labeled with DAPI (grayscale). Scale bar, 10 µm. Inset scale bar, 1 µm. (B and D) Quantification of total giantin and TGN46 area and fragment number per cell from images represented in A and C. Individual dots represent one cell and are colored by replicate ( n = 3). Bars show the median from each replicate experiment. Statistical analysis was performed using a Shapiro–Wilk normality test and a Kruskal–Wallis significance test. (E–G) Tomographic reconstructions of Golgi structures in WT (E), GMAP210 KO (F), and Golgin-160 KO (G) cells. Segmented membranes are labeled as cisternae (blue/purple), dilated structures (red), tubulovesicular structures (yellow), and vesicles (green). (E ii, F ii, iii, and G ii iii) Single-slice images with segmentation. (F ii and iii) Open arrows point to invaginations within spherical regions of tubulovesicular structures. (G ii and iii) Double-headed arrows indicate top-to-bottom fenestrations in cisternae, closed-headed arrows indicate budding structures at the nuclear envelope, and pink arrow shows frustrated budding/fusion intermediate. (E i, iii, iv, F i, iv, v, and G i, iv, v) 3D rendering of segmentation. (E–G i) Scale bar, 1 µm.

    Techniques Used: Immunolabeling, Labeling

    BGN-SBP-mSc localization in mutant cells. (A) Widefield maximum projections of cells stably expressing BGN-SBP-mSc (blue) and immunolabeled for the ERGIC (ERGIC53, green), cis/medial -Golgi (giantin, magenta), and TGN (TGN46, grayscale). Scale bar in main image, 10 µm; and insert, 1 µm. (B and C) Line-scan fluorescence intensity readings across Golgi elements measured at key time points in the RUSH assays shown in and ; and , , , , , and . The region measured is shown in inset. Line traces show (B) BGN-SBP-mSc (magenta trace) or (C) SPARC-SBP-mSc accumulates adjacent to ManII-positive Golgi elements (green trace, middle column) and then colocalizes with ManII-BFP (right column). Fluorescence measurements are normalized to the maximal point of each trace to account for differential expression and photobleaching rates of each fluorescent protein. Arrows highlight peak for each protein. ManII, mannosidase II.
    Figure Legend Snippet: BGN-SBP-mSc localization in mutant cells. (A) Widefield maximum projections of cells stably expressing BGN-SBP-mSc (blue) and immunolabeled for the ERGIC (ERGIC53, green), cis/medial -Golgi (giantin, magenta), and TGN (TGN46, grayscale). Scale bar in main image, 10 µm; and insert, 1 µm. (B and C) Line-scan fluorescence intensity readings across Golgi elements measured at key time points in the RUSH assays shown in and ; and , , , , , and . The region measured is shown in inset. Line traces show (B) BGN-SBP-mSc (magenta trace) or (C) SPARC-SBP-mSc accumulates adjacent to ManII-positive Golgi elements (green trace, middle column) and then colocalizes with ManII-BFP (right column). Fluorescence measurements are normalized to the maximal point of each trace to account for differential expression and photobleaching rates of each fluorescent protein. Arrows highlight peak for each protein. ManII, mannosidase II.

    Techniques Used: Mutagenesis, Stable Transfection, Expressing, Immunolabeling, Fluorescence, Quantitative Proteomics

    Related Articles

    Immunolabeling:

    Article Title: Multiple golgins are required to support extracellular matrix secretion, modification, and assembly
    Article Snippet: GMAP210 , BD Bioscience , BD611712 , 47539 , Full-length protein , IF 1:1,000 , PFA/MeOH.GMAP210 , BD Bioscience , BD611712 , 47539 , Full-length protein , IF 1:1,000 , PFA/MeOH.. TGN46 RRID:AB_324049 , Bio-Rad , AHP500 , 170720 , Full-length human protein , IF 1:1,000 , PFA/MeOH.. Giantin RRID:AB_291560 , Covance , PRB-114C , Clone 19243. B23348 , N terminus, residues 1–469, of human giantin , IF 1:2,000 , PFA/MeOH.Giantin RRID:AB_291560 , Covance , PRB-114C , Clone 19243. B23348 , N terminus, residues 1–469, of human giantin , IF 1:2,000 , PFA/MeOH.

    Labeling:

    Article Title: Multiple golgins are required to support extracellular matrix secretion, modification, and assembly
    Article Snippet: GMAP210 , BD Bioscience , BD611712 , 47539 , Full-length protein , IF 1:1,000 , PFA/MeOH.GMAP210 , BD Bioscience , BD611712 , 47539 , Full-length protein , IF 1:1,000 , PFA/MeOH.. TGN46 RRID:AB_324049 , Bio-Rad , AHP500 , 170720 , Full-length human protein , IF 1:1,000 , PFA/MeOH.. Giantin RRID:AB_291560 , Covance , PRB-114C , Clone 19243. B23348 , N terminus, residues 1–469, of human giantin , IF 1:2,000 , PFA/MeOH.Giantin RRID:AB_291560 , Covance , PRB-114C , Clone 19243. B23348 , N terminus, residues 1–469, of human giantin , IF 1:2,000 , PFA/MeOH.

    Mutagenesis:

    Article Title: Multiple golgins are required to support extracellular matrix secretion, modification, and assembly
    Article Snippet: GMAP210 , BD Bioscience , BD611712 , 47539 , Full-length protein , IF 1:1,000 , PFA/MeOH.GMAP210 , BD Bioscience , BD611712 , 47539 , Full-length protein , IF 1:1,000 , PFA/MeOH.. TGN46 RRID:AB_324049 , Bio-Rad , AHP500 , 170720 , Full-length human protein , IF 1:1,000 , PFA/MeOH.. Giantin RRID:AB_291560 , Covance , PRB-114C , Clone 19243. B23348 , N terminus, residues 1–469, of human giantin , IF 1:2,000 , PFA/MeOH.Giantin RRID:AB_291560 , Covance , PRB-114C , Clone 19243. B23348 , N terminus, residues 1–469, of human giantin , IF 1:2,000 , PFA/MeOH.

    Stable Transfection:

    Article Title: Multiple golgins are required to support extracellular matrix secretion, modification, and assembly
    Article Snippet: GMAP210 , BD Bioscience , BD611712 , 47539 , Full-length protein , IF 1:1,000 , PFA/MeOH.GMAP210 , BD Bioscience , BD611712 , 47539 , Full-length protein , IF 1:1,000 , PFA/MeOH.. TGN46 RRID:AB_324049 , Bio-Rad , AHP500 , 170720 , Full-length human protein , IF 1:1,000 , PFA/MeOH.. Giantin RRID:AB_291560 , Covance , PRB-114C , Clone 19243. B23348 , N terminus, residues 1–469, of human giantin , IF 1:2,000 , PFA/MeOH.Giantin RRID:AB_291560 , Covance , PRB-114C , Clone 19243. B23348 , N terminus, residues 1–469, of human giantin , IF 1:2,000 , PFA/MeOH.

    Expressing:

    Article Title: Multiple golgins are required to support extracellular matrix secretion, modification, and assembly
    Article Snippet: GMAP210 , BD Bioscience , BD611712 , 47539 , Full-length protein , IF 1:1,000 , PFA/MeOH.GMAP210 , BD Bioscience , BD611712 , 47539 , Full-length protein , IF 1:1,000 , PFA/MeOH.. TGN46 RRID:AB_324049 , Bio-Rad , AHP500 , 170720 , Full-length human protein , IF 1:1,000 , PFA/MeOH.. Giantin RRID:AB_291560 , Covance , PRB-114C , Clone 19243. B23348 , N terminus, residues 1–469, of human giantin , IF 1:2,000 , PFA/MeOH.Giantin RRID:AB_291560 , Covance , PRB-114C , Clone 19243. B23348 , N terminus, residues 1–469, of human giantin , IF 1:2,000 , PFA/MeOH.

    Fluorescence:

    Article Title: Multiple golgins are required to support extracellular matrix secretion, modification, and assembly
    Article Snippet: GMAP210 , BD Bioscience , BD611712 , 47539 , Full-length protein , IF 1:1,000 , PFA/MeOH.GMAP210 , BD Bioscience , BD611712 , 47539 , Full-length protein , IF 1:1,000 , PFA/MeOH.. TGN46 RRID:AB_324049 , Bio-Rad , AHP500 , 170720 , Full-length human protein , IF 1:1,000 , PFA/MeOH.. Giantin RRID:AB_291560 , Covance , PRB-114C , Clone 19243. B23348 , N terminus, residues 1–469, of human giantin , IF 1:2,000 , PFA/MeOH.Giantin RRID:AB_291560 , Covance , PRB-114C , Clone 19243. B23348 , N terminus, residues 1–469, of human giantin , IF 1:2,000 , PFA/MeOH.

    Quantitative Proteomics:

    Article Title: Multiple golgins are required to support extracellular matrix secretion, modification, and assembly
    Article Snippet: GMAP210 , BD Bioscience , BD611712 , 47539 , Full-length protein , IF 1:1,000 , PFA/MeOH.GMAP210 , BD Bioscience , BD611712 , 47539 , Full-length protein , IF 1:1,000 , PFA/MeOH.. TGN46 RRID:AB_324049 , Bio-Rad , AHP500 , 170720 , Full-length human protein , IF 1:1,000 , PFA/MeOH.. Giantin RRID:AB_291560 , Covance , PRB-114C , Clone 19243. B23348 , N terminus, residues 1–469, of human giantin , IF 1:2,000 , PFA/MeOH.Giantin RRID:AB_291560 , Covance , PRB-114C , Clone 19243. B23348 , N terminus, residues 1–469, of human giantin , IF 1:2,000 , PFA/MeOH.



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    tgn46 rrid ab 324049  (Bio-Rad)
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    Bio-Rad tgn46 rrid ab 324049
    Golgi organization is altered upon loss of GMAP210 or Golgin-160. (A and C) Maximum projection confocal images of WT and GMAP210 KO (A) and Golgin-160 KO (C) RPE1 cells immunolabeled for cis -Golgi (GM130, magenta), cis/medial -Golgi (giantin, green), and TGN <t>(TGN46,</t> blue) markers. Nuclei labeled with DAPI (grayscale). Scale bar, 10 µm. Inset scale bar, 1 µm. (B and D) Quantification of total giantin and <t>TGN46</t> area and fragment number per cell from images represented in A and C. Individual dots represent one cell and are colored by replicate ( n = 3). Bars show the median from each replicate experiment. Statistical analysis was performed using a Shapiro–Wilk normality test and a Kruskal–Wallis significance test. (E–G) Tomographic reconstructions of Golgi structures in WT (E), GMAP210 KO (F), and Golgin-160 KO (G) cells. Segmented membranes are labeled as cisternae (blue/purple), dilated structures (red), tubulovesicular structures (yellow), and vesicles (green). (E ii, F ii, iii, and G ii iii) Single-slice images with segmentation. (F ii and iii) Open arrows point to invaginations within spherical regions of tubulovesicular structures. (G ii and iii) Double-headed arrows indicate top-to-bottom fenestrations in cisternae, closed-headed arrows indicate budding structures at the nuclear envelope, and pink arrow shows frustrated budding/fusion intermediate. (E i, iii, iv, F i, iv, v, and G i, iv, v) 3D rendering of segmentation. (E–G i) Scale bar, 1 µm.
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    ahp500 rrid ab 324049  (Bio-Rad)
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    Golgi organization is altered upon loss of GMAP210 or Golgin-160. (A and C) Maximum projection confocal images of WT and GMAP210 KO (A) and Golgin-160 KO (C) RPE1 cells immunolabeled for cis -Golgi (GM130, magenta), cis/medial -Golgi (giantin, green), and TGN <t>(TGN46,</t> blue) markers. Nuclei labeled with DAPI (grayscale). Scale bar, 10 µm. Inset scale bar, 1 µm. (B and D) Quantification of total giantin and <t>TGN46</t> area and fragment number per cell from images represented in A and C. Individual dots represent one cell and are colored by replicate ( n = 3). Bars show the median from each replicate experiment. Statistical analysis was performed using a Shapiro–Wilk normality test and a Kruskal–Wallis significance test. (E–G) Tomographic reconstructions of Golgi structures in WT (E), GMAP210 KO (F), and Golgin-160 KO (G) cells. Segmented membranes are labeled as cisternae (blue/purple), dilated structures (red), tubulovesicular structures (yellow), and vesicles (green). (E ii, F ii, iii, and G ii iii) Single-slice images with segmentation. (F ii and iii) Open arrows point to invaginations within spherical regions of tubulovesicular structures. (G ii and iii) Double-headed arrows indicate top-to-bottom fenestrations in cisternae, closed-headed arrows indicate budding structures at the nuclear envelope, and pink arrow shows frustrated budding/fusion intermediate. (E i, iii, iv, F i, iv, v, and G i, iv, v) 3D rendering of segmentation. (E–G i) Scale bar, 1 µm.
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    Image Search Results


    Golgi organization is altered upon loss of GMAP210 or Golgin-160. (A and C) Maximum projection confocal images of WT and GMAP210 KO (A) and Golgin-160 KO (C) RPE1 cells immunolabeled for cis -Golgi (GM130, magenta), cis/medial -Golgi (giantin, green), and TGN (TGN46, blue) markers. Nuclei labeled with DAPI (grayscale). Scale bar, 10 µm. Inset scale bar, 1 µm. (B and D) Quantification of total giantin and TGN46 area and fragment number per cell from images represented in A and C. Individual dots represent one cell and are colored by replicate ( n = 3). Bars show the median from each replicate experiment. Statistical analysis was performed using a Shapiro–Wilk normality test and a Kruskal–Wallis significance test. (E–G) Tomographic reconstructions of Golgi structures in WT (E), GMAP210 KO (F), and Golgin-160 KO (G) cells. Segmented membranes are labeled as cisternae (blue/purple), dilated structures (red), tubulovesicular structures (yellow), and vesicles (green). (E ii, F ii, iii, and G ii iii) Single-slice images with segmentation. (F ii and iii) Open arrows point to invaginations within spherical regions of tubulovesicular structures. (G ii and iii) Double-headed arrows indicate top-to-bottom fenestrations in cisternae, closed-headed arrows indicate budding structures at the nuclear envelope, and pink arrow shows frustrated budding/fusion intermediate. (E i, iii, iv, F i, iv, v, and G i, iv, v) 3D rendering of segmentation. (E–G i) Scale bar, 1 µm.

    Journal: The Journal of Cell Biology

    Article Title: Multiple golgins are required to support extracellular matrix secretion, modification, and assembly

    doi: 10.1083/jcb.202411167

    Figure Lengend Snippet: Golgi organization is altered upon loss of GMAP210 or Golgin-160. (A and C) Maximum projection confocal images of WT and GMAP210 KO (A) and Golgin-160 KO (C) RPE1 cells immunolabeled for cis -Golgi (GM130, magenta), cis/medial -Golgi (giantin, green), and TGN (TGN46, blue) markers. Nuclei labeled with DAPI (grayscale). Scale bar, 10 µm. Inset scale bar, 1 µm. (B and D) Quantification of total giantin and TGN46 area and fragment number per cell from images represented in A and C. Individual dots represent one cell and are colored by replicate ( n = 3). Bars show the median from each replicate experiment. Statistical analysis was performed using a Shapiro–Wilk normality test and a Kruskal–Wallis significance test. (E–G) Tomographic reconstructions of Golgi structures in WT (E), GMAP210 KO (F), and Golgin-160 KO (G) cells. Segmented membranes are labeled as cisternae (blue/purple), dilated structures (red), tubulovesicular structures (yellow), and vesicles (green). (E ii, F ii, iii, and G ii iii) Single-slice images with segmentation. (F ii and iii) Open arrows point to invaginations within spherical regions of tubulovesicular structures. (G ii and iii) Double-headed arrows indicate top-to-bottom fenestrations in cisternae, closed-headed arrows indicate budding structures at the nuclear envelope, and pink arrow shows frustrated budding/fusion intermediate. (E i, iii, iv, F i, iv, v, and G i, iv, v) 3D rendering of segmentation. (E–G i) Scale bar, 1 µm.

    Article Snippet: TGN46 RRID:AB_324049 , Bio-Rad , AHP500 , 170720 , Full-length human protein , IF 1:1,000 , PFA/MeOH.

    Techniques: Immunolabeling, Labeling

    BGN-SBP-mSc localization in mutant cells. (A) Widefield maximum projections of cells stably expressing BGN-SBP-mSc (blue) and immunolabeled for the ERGIC (ERGIC53, green), cis/medial -Golgi (giantin, magenta), and TGN (TGN46, grayscale). Scale bar in main image, 10 µm; and insert, 1 µm. (B and C) Line-scan fluorescence intensity readings across Golgi elements measured at key time points in the RUSH assays shown in and ; and , , , , , and . The region measured is shown in inset. Line traces show (B) BGN-SBP-mSc (magenta trace) or (C) SPARC-SBP-mSc accumulates adjacent to ManII-positive Golgi elements (green trace, middle column) and then colocalizes with ManII-BFP (right column). Fluorescence measurements are normalized to the maximal point of each trace to account for differential expression and photobleaching rates of each fluorescent protein. Arrows highlight peak for each protein. ManII, mannosidase II.

    Journal: The Journal of Cell Biology

    Article Title: Multiple golgins are required to support extracellular matrix secretion, modification, and assembly

    doi: 10.1083/jcb.202411167

    Figure Lengend Snippet: BGN-SBP-mSc localization in mutant cells. (A) Widefield maximum projections of cells stably expressing BGN-SBP-mSc (blue) and immunolabeled for the ERGIC (ERGIC53, green), cis/medial -Golgi (giantin, magenta), and TGN (TGN46, grayscale). Scale bar in main image, 10 µm; and insert, 1 µm. (B and C) Line-scan fluorescence intensity readings across Golgi elements measured at key time points in the RUSH assays shown in and ; and , , , , , and . The region measured is shown in inset. Line traces show (B) BGN-SBP-mSc (magenta trace) or (C) SPARC-SBP-mSc accumulates adjacent to ManII-positive Golgi elements (green trace, middle column) and then colocalizes with ManII-BFP (right column). Fluorescence measurements are normalized to the maximal point of each trace to account for differential expression and photobleaching rates of each fluorescent protein. Arrows highlight peak for each protein. ManII, mannosidase II.

    Article Snippet: TGN46 RRID:AB_324049 , Bio-Rad , AHP500 , 170720 , Full-length human protein , IF 1:1,000 , PFA/MeOH.

    Techniques: Mutagenesis, Stable Transfection, Expressing, Immunolabeling, Fluorescence, Quantitative Proteomics